Additionally, the 90-day seeds seemed to have germinated well even though the seed coat was left intact. I don't know how crucial the removal of the seed coat is to germination. I think a properly stratified seed with optimal environmental conditions will germinate just fine with or without the seed coat. I may do some experiments next year with seed coat v.s. Non seed coat germination. But I believe I will leave most of the seed coats on for next year's germination.
In summary, thus far my best practices for asimina triloba seed germination are as follows.
1. Immediately clean seeds removed from fruit.
2. Immediately wrap seeds in moist paper towel.
3. Double-bag seeds/paper towel in plastic food storage bag to conserve moisture.
4. Leave seeds in refrigerator for 90 days minimum, changing the paper towel every few weeks to prevent mold and fungus.
5. Place seeds in 10-inch tree pots containing moist soil.
6. Keep tree pots in a plastic tote with lid to increase moisture and humidity.
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